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The Zanetti lab

Cryo-tomography of coats


Please visit ORCID for a full list.

Pyle E, Zanetti G. Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes 2024-01-17, BiorXiv, DOI: 10.1101/2024.01.17.576008

Van der Verren SE, Zanetti G. The small GTPase Sar1, control centre of COPII trafficking FEBS Letters 2023-03 DOI: 10.1002/1873-3468.14595

Zivanov J, Otón J, Ke Z, von Kügelgen A, Pyle E, Qu K, Morado D, Castaño-Díez D, Zanetti G, Bharat T, Briggs J, Scheres S. A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0. eLife 2022-12-05 DOI: 10.7554/eLife.83724

Pyle E, Hutchings J, Zanetti G. Strategies for picking membrane-associated

particles within subtomogram averaging workflows. Faraday Discuss. 2022 Aug 4.

doi: 10.1039/d2fd00022a. Epub ahead of print. PMID: 35924570.


Greiwe JF, Zanetti G, Miller TCR, Costa A. In silico reconstitution of DNA

replication. Lessons from single-molecule imaging and cryo-tomography applied to

single-particle cryo-EM. Curr Opin Struct Biol. 2022 Feb;72:279-286. doi:

10.1016/ Epub 2022 Jan 10. PMID: 35026552; PMCID: PMC8869182.


Conley MJ, Short JM, Burns AM, Streetley J, Hutchings J, Bakker SE, Power BJ,

Jaffery H, Haney J, Zanetti G, Murcia PR, Stewart M, Fearns R, Vijayakrishnan S,

Bhella D. Helical ordering of envelope-associated proteins and glycoproteins in

respiratory syncytial virus. EMBO J. 2022 Feb 1;41(3):e109728. doi:

10.15252/embj.2021109728. Epub 2021 Dec 22. PMID: 34935163; PMCID: PMC8804925.


Pyle E, Zanetti G. Current data processing strategies for cryo-electron

tomography and subtomogram averaging. Biochem J. 2021 May 28;478(10):1827-1845.

doi: 10.1042/BCJ20200715. PMID: 34003255; PMCID: PMC8133831.


Hutchings J, Stancheva VG, Brown NR, Cheung ACM, Miller EA, Zanetti G.

Structure of the complete, membrane-assembled COPII coat reveals a complex

interaction network. Nat Commun. 2021 Apr 1;12(1):2034. doi:

10.1038/s41467-021-22110-6. PMID: 33795673; PMCID: PMC8016994.


Stancheva VG, Li XH, Hutchings J, Gomez-Navarro N, Santhanam B, Babu MM,

Zanetti G, Miller EA. Combinatorial multivalent interactions drive cooperative

assembly of the COPII coat. J Cell Biol. 2020 Nov 2;219(11):e202007135. doi:

10.1083/jcb.202007135. PMID: 32997735; PMCID: PMC7594496.


Castaño-Díez D, Zanetti G. In situ structure determination by subtomogram

averaging. Curr Opin Struct Biol. 2019 Oct;58:68-75. doi:

10.1016/ Epub 2019 Jun 21. PMID: 31233977; PMCID: PMC7116126.


Hutchings J, Zanetti G. Coat flexibility in the secretory pathway: a role in

transport of bulky cargoes. Curr Opin Cell Biol. 2019 Aug;59:104-111. doi:

10.1016/ Epub 2019 May 21. PMID: 31125831; PMCID: PMC7116127.


Markova EA, Zanetti G. Visualizing membrane trafficking through the electron

microscope: cryo-tomography of coat complexes. Acta Crystallogr D Struct Biol.

2019 May 1;75(Pt 5):467-474. doi: 10.1107/S2059798319005011. Epub 2019 Apr 30.

PMID: 31063149; PMCID: PMC6503763.

Hutchings J, Stancheva V, Miller EA, Zanetti G. Subtomogram averaging of

COPII assemblies reveals how coat organization dictates membrane shape. Nat

Commun. 2018 Oct 8;9(1):4154. doi: 10.1038/s41467-018-06577-4. PMID: 30297805;

PMCID: PMC6175875.


Hutchings J, Zanetti G. Fine details in complex environments: the power of

cryo-electron tomography. Biochem Soc Trans. 2018 Aug 20;46(4):807-816. doi:

10.1042/BST20170351. Epub 2018 Jun 22. Erratum in: Biochem Soc Trans. 2018 Dec

17;46(6):1771. PMID: 29934301; PMCID: PMC6103461.


Ilangovan A, Kay CWM, Roier S, El Mkami H, Salvadori E, Zechner EL, Zanetti

G, Waksman G. Cryo-EM Structure of a Relaxase Reveals the Molecular Basis of DNA

Unwinding during Bacterial Conjugation. Cell. 2017 May 4;169(4):708-721.e12.

doi: 10.1016/j.cell.2017.04.010. Epub 2017 Apr 27. PMID: 28457609; PMCID:



Previous publications

Zanetti G, Prinz S, Daum S, Meister A, Schekman R, Bacia K, Briggs JA. The

structure of the COPII transport-vesicle coat assembled on membranes. Elife.

2013 Sep 17;2:e00951. doi: 10.7554/eLife.00951. PMID: 24062940; PMCID:



Guo Y, Zanetti G, Schekman R. A novel GTP-binding protein-adaptor protein

complex responsible for export of Vangl2 from the trans Golgi network. Elife.

2013 Jan 8;2:e00160. doi: 10.7554/eLife.00160. Erratum in: Elife. 2013;2:e01328.

PMID: 23326640; PMCID: PMC3539332.


Zanetti G, Pahuja KB, Studer S, Shim S, Schekman R. COPII and the regulation

of protein sorting in mammals. Nat Cell Biol. 2011 Dec 22;14(1):20-8. doi:

10.1038/ncb2390. Erratum in: Nat Cell Biol. 2012 Feb;14(2):221. PMID: 22193160.


Zanetti G, Riches JD, Fuller SD, Briggs JA. Contrast transfer function

correction applied to cryo-electron tomography and sub-tomogram averaging. J

Struct Biol. 2009 Nov;168(2):305-12. doi: 10.1016/j.jsb.2009.08.002. Epub 2009

Aug 8. PMID: 19666126; PMCID: PMC2806944.


Zanetti G, Briggs JA, Grünewald K, Sattentau QJ, Fuller SD. Cryo-electron

tomographic structure of an immunodeficiency virus envelope complex in situ.

PLoS Pathog. 2006 Aug;2(8):e83. doi: 10.1371/journal.ppat.0020083. PMID:

16933990; PMCID: PMC1557830.


van Thor JJ, Zanetti G, Ronayne KL, Towrie M. Structural events in the

photocycle of green fluorescent protein. J Phys Chem B. 2005 Aug

25;109(33):16099-108. doi: 10.1021/jp051315+. PMID: 16853046.


Staunton D, Schlinkert R, Zanetti G, Colebrook SA, Campbell ID. Cell-free

expression and selective isotope labelling in protein NMR. Magn Reson Chem. 2006

Jul;44 Spec No:S2-9. doi: 10.1002/mrc.1835. PMID: 16826537.


Sasson SC, Zaunders JJ, Zanetti G, King EM, Merlin KM, Smith DE, Stanley KK,

Cooper DA, Kelleher AD. Increased plasma interleukin-7 level correlates with

decreased CD127 and Increased CD132 extracellular expression on T cell subsets

in patients with HIV-1 infection. J Infect Dis. 2006 Feb 15;193(4):505-14. doi:

10.1086/499309. Epub 2006 Jan 17. PMID: 16425129.

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