The Zanetti lab

Cryo-tomography of coats

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Please visit ORCID for a full list.

Check out our BioRxiv pre-prints on COPII complex interaction network.

Great collaboration with Liz Miller's lab at the LMB.

 

Hutchings, J. et al. Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network. 

Recent papers from the lab

·      Stancheva, V. G., Li X., Hutchings, J., Gomez-Navarro, N., Santhanam, B., Babu, M. M., Zanetti, G., & Miller, E. A. Combinatorial multivalent interactions drive cooperative assembly of the COPII coat. J. Cell Biol. 2, 219 (2020).

·      Hutchings, J. & Zanetti, G. Coat flexibility in the secretory pathway: a role in transport of bulky cargoes. Current Opinion in Cell Biology 59, 104–111 (2019).

·      Markova, E. A. & Zanetti, G. Visualizing membrane trafficking through the electron microscope: cryo-tomography of coat complexes. Acta Crystallogr D Struct Biol 75, 467–474 (2019).

·      Castaño-Díez, D. & Zanetti, G. In situ structure determination by subtomogram averaging. Curr. Opin. Struct. Biol. 58, 68–75 (2019).

·      Hutchings, J., Stancheva, V., Miller, E. A. & Zanetti, G. Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape. Nature Communications 9, 4154 (2018).

·      Hutchings, J. & Zanetti, G. Fine details in complex environments: the power of cryo-electron tomography. Biochem. Soc. Trans. 46, 807–816 (2018).

·      Ilangovan, A. et al. Cryo-EM Structure of a Relaxase Reveals the Molecular Basis of DNA Unwinding during Bacterial Conjugation. Cell 169, 708-721.e12 (2017).