The Zanetti lab

Cryo-tomography of coats

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Please visit ORCID for a full list.

Recent papers from the lab

Pyle, E., Zanetti, G. Current data processing strategies for cryo-electron tomography and subtomogram averaging. Biochem J., (2021).

Hutchings, J., Stancheva, V. G., Brown, N. R., Cheung, A. C. M., Miller, E. A. and Zanetti, G. Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network. Nature Communications 12, 2034 (2021)

·      Stancheva, V. G., Li X., Hutchings, J., Gomez-Navarro, N., Santhanam, B., Babu, M. M., Zanetti, G., & Miller, E. A. Combinatorial multivalent interactions drive cooperative assembly of the COPII coat. J. Cell Biol. 2, 219 (2020).

·      Hutchings, J. & Zanetti, G. Coat flexibility in the secretory pathway: a role in transport of bulky cargoes. Current Opinion in Cell Biology 59, 104–111 (2019).

·      Markova, E. A. & Zanetti, G. Visualizing membrane trafficking through the electron microscope: cryo-tomography of coat complexes. Acta Crystallogr D Struct Biol 75, 467–474 (2019).

·      Castaño-Díez, D. & Zanetti, G. In situ structure determination by subtomogram averaging. Curr. Opin. Struct. Biol. 58, 68–75 (2019).

·      Hutchings, J., Stancheva, V., Miller, E. A. & Zanetti, G. Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape. Nature Communications 9, 4154 (2018).

·      Hutchings, J. & Zanetti, G. Fine details in complex environments: the power of cryo-electron tomography. Biochem. Soc. Trans. 46, 807–816 (2018).

·      Ilangovan, A. et al. Cryo-EM Structure of a Relaxase Reveals the Molecular Basis of DNA Unwinding during Bacterial Conjugation. Cell 169, 708-721.e12 (2017).